Journal: Environmental and molecular mutagenesis
Article Title: Flow cytometric method for scoring rat liver micronuclei with simultaneous assessments of hepatocyte proliferation
doi: 10.1002/em.22168
Figure Lengend Snippet: a-f. Flow cytometric data for the 3 day diethylnitrosamine study are shown. Panel a = Change in body weight over the course of the treatment period; panel b = normalized liver weights; panel c = %MNHEP; panel d = %Ki-67-positive nuclei; panel e = hepatocyte proliferation index; panel f = %8n+ nuclei. For this study, low, mid, and high treatment groups correspond to 10, 20, and 40 mg/kg/day, respectively. For every graph, data for each individual rat is shown, and group means appear as horizontal green lines. Dunnett’s test results are shown to the far right of each graph, where statistically significant differences relative to the concurrent vehicle control group appear as italicized black text as opposed to red text, and by grey circles as opposed to red circles. Circles’ diameters represent 95% confidence intervals.
Article Snippet: Heat-inactivated fetal bovine serum (FBS; cat. no. 89510-186) was from VWR, Radnor, PA. Reagents used for flow cytometric MN-RET scoring (Anticoagulant Solution, Buffer Solution, DNA Stain, Anti-CD71-FITC and Anti-CD61-PE Antibodies, RNase Solution, and Malaria Biostandards) were from In Vivo MicroFlow ® PLUS R Kits, Litron Laboratories, Rochester, NY.
Techniques: